Total mRNA were extracted from homogenized liver and subcutaneous white adipose tissue samples using a Precellys tissue homogenizer Bertin Technology, Saint Quentin en Yveline, France and the GenElute Mammalian Total RNA Miniprep kit Sigma Aldrich, then reverse transcribed using the High Capacity cDNA Reverse Transcription kit Applied Biosystems, Foster City, CA <a href=http://buycialis.buzz>cialis generic cost</a>
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